Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences CAPS >=99% | 1135-40-6 | MFCD00003837 | 6X100G
CAPS >=99% | Purity: >=99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 6X100G
Medchemexpress LLC 4-Morpholineethanesulfonic acid, potassium salt | 39946-25-3 | 99.9% | 10 G
MES potassium (2-Morpholinoethanesulphonic acid potassium) is an amphoteric ion buffer with a buffering capacity ranging from pH 5.5-7.0. As a Good's buffer, it is utilized in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies. This zwitterionic buffer provides effective buffering capacity around pH 6.15 and shows poor affinity with metal ions, making it suitable for studies involving them.
- Amphoteric ion buffer
- Buffer capacity ranging pH 5.5-7.0
- Widely used in biochemistry and molecular biology experiments
- Effective buffering capacity at around pH 6.15
- Poor affinity with metal ions, making it suitable for studies involving metal ions
eMolecules tert-butyl 3-(hydroxymethyl)-3-methylpiperidine-1-carboxylate | 406212-48-4 | MFCD21642046 | 1g
Pharmablock | tert-butyl 3-(hydroxymethyl)-3-methylpiperidine-1-carboxylate | 1g | 551129390 | PBN2011457 | | 406212-48-4 | MFCD21642046 | 229.320 | C12H23NO3
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Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.8 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.8 (0.2 micron filtered), 500 ml.
Boston Bioproducts Inc Tris Buffered Saline, TBS (10X, pH 7.4, Low Endotoxin) - 100ml
The Tris Buffered Saline (TBS) is an isotonic non-toxic buffer solution used commonly in biochemical immunoassay as a wash buffer. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 10X concentration and tested for endotoxin.
eMolecules tert-butyl 4-(hydroxymethyl)pyrazole-1-carboxylate | 199003-22-0 | MFCD24335255 | 1g
Pharmablock | tert-butyl 4-(hydroxymethyl)pyrazole-1-carboxylate | 1g | 551300363 | PBXA8097 | | 199003-22-0 | MFCD24335255 | 198.222 | C9H14N2O3
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Medchemexpress LLC PbS/CdS QDs (PbS/CdSe QD) | PbS/CdS | 10 MG
PbS/CdS quantum dots are colloidal nanocrystals designed as a fluorescent probe for in vivo imaging in the second near-infrared (NIR-II) window. These aqueous suspensions emit near ~1300 nm, yielding high signal-to-background ratio and reduced tissue scattering for deeper, clearer fluorescence imaging. The product is supplied as a light yellow to brown liquid suspension at 10 mg/mL and requires protection from light during storage.