Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 250 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660241.jpg-250.jpg)
Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 250 G
HEPES, Sodium Salt is a biochemical reagent supplied in a 250g package, offering a reliable buffer for experiments requiring precise pH control. This sodium salt form of HEPES maintains a stable pH of 6.8–8.2, which is essential for a variety of biochemical and biological applications such as cell culture, protein analysis, and enzymatic assays. HEPES sodium salt is known for its excellent buffering capacity, ensuring that pH remains constant even during extended incubation periods. It is widely used in research and laboratory environments due to its low toxicity, making it a preferred choice for maintaining stable conditions during biological experiments.
![Alkali Scientific HEPPSO [N (2 Hydroxyethyl) piperazine N' (2 hydroxypropanesulfonic acid)], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661332.jpg-250.jpg)
Alkali Scientific HEPPSO [N (2 Hydroxyethyl) piperazine N' (2 hydroxypropanesulfonic acid)], 100 G
HEPPSO (N-(2 Hydroxyethyl) piperazine N'-(2 hydroxypropanesulfonic acid)) is a biochemical reagent supplied in a 100g bottle. This buffering agent is commonly used in laboratories for maintaining a stable pH, particularly in molecular biology and biochemistry applications. HEPPSO is widely used for enzyme assays, protein purification, and chromatography, as well as in the preparation of biological samples where precise pH control is necessary. This high-quality reagent ensures consistent results in experiments that require a stable and controlled acidic environment, especially in cell culture, electrophoresis, and other biological assays.
Medchemexpress LLC 3-morpholino-2-hydroxypropanesulfonic acid | 68399-77-9 | MFCD00009629 | 99.7% | 225.26 g/mol | C7H15NO5S | 500 G
MOPSO is a zwitterionic biological buffer used to maintain stable near-neutral pH in biochemical and cell culture applications. It buffers effectively in the 6.2-7.6 pH range and is supplied as a high-purity powder with supporting analytical documentation for quality control.
- Useful pH range of 6.2-7.6.
- High purity suitable for research applications.
- Appropriate for biological and biochemical buffering needs.
- Supplied as a powder for convenient storage and handling.
- Analytical documentation available (COA, SDS, HNMR, RP-HPLC, MS).
BIOHIPPO Research Grade Anti-Human IL10 Biosimilar Antibody (MK-1966), 100 µg
MK-1966 is a research-grade biosimilar antibody targeting IL10. It reacts with Human. Suitable for ELISA, FACS, Functional assay, Research in vivo. This research-grade biosimilar supports comparability, functional, PK/PD and immunogenicity studies. Supplied as 100 ug.
Chem-Impex International, Inc. 3-Cyclohexylamino-1-propanesulfonic acid | 1135-40-6 | MFCD00003837 | 100G
3-Cyclohexylamino-1-propanesulfonic acid, 1135-40-6, MFCD00003837, 100G
Alkali Scientific Murashige & Skoog Medium with MES Buffer, 240 Grams of Powder, Makes 50 Liters of Solution
Murashige & Skoog (MS) Medium with MES Buffer is a powdered medium formulated for plant tissue culture applications. The 240g powder makes 50 liters of nutrient solution, enriched with MES buffer to stabilize the pH during plant cell culture. The inclusion of MES ensures better pH stability during long incubation periods, especially in environments where pH fluctuations can affect cell growth. This medium is ideal for research in plant biotechnology, as it supports both the growth and differentiation of plant cells, tissues, and organs. It is commonly used for in vitro studies, propagation, and transformation of various plant species.
Sigma Aldrich Fine Chemicals Biosciences BICINE BioXtra 99 titratio250G
BICINE BioXtra 99 titratio250G
Medchemexpress LLC L-Adenosine | 3080-29-3 | 99.8% | 1 ML
L-Adenosine is a metabolically stable enantiomeric analog and a potential probe. It exhibits weakly inhibitory adenosine deaminase (ADA) activity with a Ki value of 385 μM. This compound can be utilized for research into adenosine uptake and accumulation.
- Metabolically stable enantiomeric analog.
- Potential probe for research.
- Weakly inhibits adenosine deaminase activity.
- Can be used for adenosine uptake research.
- Can be used for adenosine accumulation research.
![Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661238.jpg-250.jpg)
Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 5 Kilograms
PIPES Sesquisodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) sesquisodium salt), 5 Kilograms, is a highly specialized biochemical reagent used primarily for preparing buffers in laboratory research. PIPES is particularly valuable for maintaining a stable pH in biological systems, including enzyme reactions and cell culture environments. It is commonly used in electrophoresis, chromatography, and as a component in molecular biology protocols. The large 5 kg volume makes it ideal for high-throughput laboratories and large-scale biochemical experiments. PIPES is known for its exceptional buffering capacity in the physiological pH range, making it an essential tool in many laboratory applications.