Life Science Buffers
Apexbio Technology LLC TUNEL Apoptosis Detection Kit (DAB) 20rxns
The TUNEL Apoptosis Detection Kit (DAB) is designed to detect nuclear DNA fragmentation occurring during apoptosis in tissues or cells During apoptosis endonucleases within cells become activated and cleave genomic DNA between nucleosomes resulting in DNA fragments of approximately 180-200 bp or multiples thereof The fragmented DNA generates free 3 -OH termini at which terminal deoxynucleotidyl transferase (TdT) incorporates biotin-labeled dUTP Subsequently these labeled termini bind to horseradish peroxidase (HRP)-conjugated streptavidin (Streptavidin-HRP) followed by chromogenic visualization using DAB This kit allows direct observation using an ordinary optical microscope and is convenient for use It is applicable to a wide range of sample types including frozen sections paraffin-embedded sections adherent cell cultures and suspended cells making it versatile for apoptosis detection
Apexbio Technology LLC Deacetylase Inhibitor Cocktail (100X in 70% DMSO) 100x1ml
Deacetylase Inhibitor Cocktail is composed of several compounds that act synergistically to maintain the acetylation status of molecules The balance between acetyltransferase and deacetylase activities impacts chromatin compaction transcription factor activity and protein aggregation and the variations of these modifications have garnered considerable interest from researchers Our product Deacetylase Inhibitor Cocktail is a valuable tool for investigating these changes The Deacetylation Inhibition Cocktail includes four active components 40 M Trichostatin A 1 mM EX-527 400 mM nicotinamide and 200 mM sodium butyrate These compounds are dissolved in 70% DMSO each with distinct specific actions Trichostatin A inhibits class I/II HDACs EX-527 selectively inhibits SIRT1 with weaker activity against SIRT2 and SIRT3 Nicotinamide acts as an inhibitor of sirtuins (SIRT1-7 class III HDACs) Sodium butyrate competitively inhibits class I/II HDACs
Apexbio Technology LLC EdU Flow Cytometry Assay Kits (HF488) 100tests
EdU Flow Cytometry Assay Kits (HF488) provides a novel method based on click chemistry specifically CuAAC (copper-catalyzed azide-alkyne cycloaddition) enabling direct measurement of DNA synthesis during the S-phase of the cell cycle The thymidine analog EdU (5-ethynyl-2 -deoxyuridine) can be incorporated directly into DNA strands during DNA synthesis The alkyne group on EdU a biologically inert moiety undergoes highly selective reaction with azide-functionalized dyes via the CuAAC reaction forming a stable 1 2 3-triazole product The EdU Flow Cytometry Assay Kits (HF488) use HyperFluor 488 azide to label the DNA of proliferating cells following its reaction with EdU Samples labeled in this manner can be analyzed via flow cytometry (HyperFluor 594 azide excitation maximum at 496 nm emission maximum at 516 nm)
Apexbio Technology LLC Annexin V-PE/DAPI Apoptosis Kit 20tests
Annexin V-PE/DAPI Apoptosis Kit enables differentiation between early apoptotic late apoptotic and dead cells Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane phospholipid bilayer However during the early stages of apoptosis PS moves from the inner surface to the outer surface of the cell membrane becoming exposed to the extracellular environment Labeled with a fluorescence dye such as PE Annexcin V is commonly used as a fluorescent probe to detect apoptosis through flow cytometry or fluorescence microscopy within 15-30 minutes Additionally this kit includes the nuclear stain DAPI which specifically binds to double-stranded DNA to emit blue fluorescence Normally DAPI cannot penetrate intact cell membranes In late apoptotic and dead cells DAPI can cross the compromised membrane to stain the nucleus
Boston Bioproducts Inc Tris Buffered Saline, TBS (10X, pH 7.4) - 4L
The Tris Buffered Saline (TBS) is an isotonic non-toxic buffer solution used commonly in biochemical immunoassay as a wash buffer. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 10X concentration.
Teknova DNA Suspension Buffer, pH 8.0. 4L, Sterile, DNase/RNase tested, PCR Grade. 2 pack.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
Teknova CTAB Extraction Solution. 250mL Bottle. 4 pack.
2.0% CTAB, 100mM Tris-HCl, pH 8.0,20mM EDTA, pH 8.0, 1.4M Sodium Chloride
Teknova DNA Suspension Buffer, pH 8.0, 10L, DNase/RNase Tested, PCR Grade, Sterile. 2 Pack.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
Teknova 20X PBS with 1% Tween-20. 500mL, Sterile. 8 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Teknova 1X Dulbeccos Phosphate Buffered Saline with 0.5% BSA. 1000mL, Sterile. 2 pack.
136.89mM Sodium Chloride, 8.10mM Disodium Phosphate, 2.68mM Potassium Chloride,1.47mM Monopotassium Phosphate, 0.5% Bovine Serum Albumin (BSA)
Teknova 10mM Tris-HCl, pH 8.5. 2mL, 100-Pack, Sterile. 2 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
